LFA-1, an antigen associated with antigen-specific T lymphocyte-mediated killing, and Mac-1, a macrophage differentiation antigen associated with type three complement receptor function, contain alpha chains of Mr = 180,000 and 170,000, respectively, and beta chains of Mr = 95,000. The monoclonal antibodies defining these antigens do not cross-react. The LFA-1 and Mac-1 beta chains are highly homologous or identical, whereas the alpha chains are highly different by tyrosyl tryptic peptide mapping (Kürzinger, K., Ho, M. K., and Springer, T. A. (1982) Nature (Lond.) 296, 668-670). T lymphoma cell lines express LFA-1 but not Mac-1 as shown by immunofluorescence and immunoprecipitation. Conversely, some macrophage-like lines express Mac-1 but not LFA-1. Other macrophage-like lines co-express Mac-1 and small amounts of LFA-1. Mac-1 and LFA-1 are present as separate molecules in these cells. [35S]Methionine and [[3H]glucosamine are incorporated into both alpha and beta chains of Mac-1 and LFA-1, showing both chains are endogenously synthesized and are glycoproteins. Cross-linking and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis experiments show that in both Mac-1 and LFA-1 the alpha and beta chains are noncovalently associated in alpha 1 beta 1 quaternary structures. By quantitative immunofluorescence flow cytometry, the EL-4 T lymphoma and P388D1 macrophage-like lines were estimated to express 10(5) LFA-1 and 1.6 x 10(5) Mac-1 molecules/cell, respectively. From these sources the antigens have been purified to homogeneity in 200-400-micrograms quantities by monoclonal antibody affinity chromatography. The purified antigens contain only the alpha and beta subunits.
Publications
Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen. J. Biol. Chem. 257, 20, 12412-12418 (1982).Abstract
Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor. J. Exp. Med. 156, 4, 1000-1009 (1982).Abstract
Structural homology of a macrophage differentiation antigen and an antigen involved in T-cell-mediated killing. Nature 296, 5858, 668-670 (1982).
Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell. Immunol. 73, 1, 1-11 (1982).
Macrophage differentiation antigens: markers for macrophage subpopulations and tissue localization. Hybridomas in cancer diagnosis and treatment 53-61 (1982).
Mac-1 antigen: Quantitative expression in macrophage populations and tissues, and immunofluorescent localization in spleen. J. Immunol. 128, 2281-2286 (1982).
Murine macrophage differentiation antigens defined by monoclonal antibodies. Monoclonal hybridoma antibodies: techniques and applications 169-175 (1982).
Three distinct antigens associated with human T lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc Natl Acad Sci USA 79, 23, 7489-7493 (1982).Abstract
A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J. Immunol. 127, 6, 2488-2495 (1981).Abstract
Cross reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J. Immunol. 126, 1, 359-364 (1981).Abstract
Characterization of an anti-H-2 monoclonal antibody and its use in large-scale antigen purification. J. Immunol. 127, 3, 923-930 (1981).Abstract
Rat anti-mouse macrophage monoclonal antibodies and their use in immunofluorescent studies of macrophages in tissue sections. Monoclonal antibodies and T cell hybridomas 53-61 (1981).
Use of a monoclonal antibody specifically non-reactive with T cells to delineate lymphocyte subpopulations. Immunology 42, 3, 371-378 (1981).Abstract
Monoclonal antibodies as tools for the study of mononuclear phagocytes. Methods for studying mononuclear phagocytes 305-313 (1981).
Monoclonal antibodies as probes of surface structures participating in T-lymphocyte function. Monoclonal antibodies and T cell hybridomas 45-52 (1981).
A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and structure. J. Immunol. 127, 2, 596-602 (1981).Abstract