Protein Expression
- Establishment of Stable Transfectant of CHO Lec Cells (J. Takagi)
- Transient Transfection into 293T Cells (J. Takagi)
- Preparing a Selenomethionyl Protein (S. Lindgren)
Protein Purification, Characterization
- Sepharose Affinity Gels (T. Springer)
- Purification of ICAM-1 (M. Ferzly)
- SDS Slab Gel Electrophoresis (T. Springer)
- Preparation of Tonsil Lysate (M. Ferzly)
- [Sandwich] ELISA for Detecting Secreted ICAM-1 (C-D. Jun)
- Purification of Rat Monoclonal Antibodies (T. Springer)
Radioactive Experiments
- Bench Top Radioactive Work Protocol (M. Ferzly)
- Radioiodination (J. Takagi)
- Metabolic Labeling and Immunoprecipitation (J. Takagi)
- Internal radiolabeling of monoclonal immunoglobulins (T. Springer)
- High Specific Activity 3H-labeled Ig for Cross-Inhibition experiments (T. Springer)
- Protein Iodination (T. Springer)
- Anti-Ig Indirect Assay for mAb binding to Cell Surface Antigens (T. Springer)
- Immunoprecipitation for Analysis of Radiolabeled Cell Surface Molecules (T. Springer)
-
Polyacrylamide Fluorography (T. Springer)
Immunochemical Methods
- Triple Sandwich Immunoprecipitation System (F. Sanchez-Madrid)
- Immunoblotting on Nitrocellulose (F. Sanchez Madrid)
- Conjugation of protein to FITC (T. Springer)
- Semi-Quantitative Measurement of Proteins by Dot Blotting (J. Takagi)
- Purification of mAb (IgG) (C-D. Jun)
- Direct Immunofluorescence Staining of Cell Surfaces in Whole Blood (Becton Dickinson)
-
Quantitative Single Radial Immunodiffusion (T. Springer)
Cell Biology Protocols
- Purification of Mononuclear Cells and Neutrophils on Density Gradients (T. Springer)
- Purification of Human Mononuclear Cells and Neutrophils (C-D. Jun)
- Separation of Platelets from Whole Blood (A. Salas)
- L-selectin-PNAd interactions under flow conditions. Flow Chamber Assay (A. Salas)
- FACS Analysis Protocols (T. Springer)
- Paraformaldehyde Fixation of Fluorescent Stained Hematopoietic Cells (Becton Dickinson)
Hybridomas, Cell Culture
- Cell Hybridization Using PEG (T. Springer)
- Freezing Cells, Liquid N2 storage (T. Springer)
- Chemotaxis Assay (M. Swope)
- Soft agar cloning (T. Springer)
- Large scale media prep (T. Springer)
Cloning/Molecular Techniques
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Lentiviral transduction (T. Schurpf)